FIG 3.

Small interfering RNA silencing of Wnt pathway receptor complex proteins significantly reduces E. chaffeensis infection. Small interfering RNA-transfected THP-1 cells were infected or mock infected with E. chaffeensis (MOI 100, 24 h posttransfection). (A) Infection level was quantified at 24 hpi by qPCR amplification of ehrlichial dsb for each siRNA treatment (y axis). Scrambled siRNA (scrRNA) was transfected as a negative control. Values represent the mean plus or minus standard deviation. *, P < 0.05; ns, P > 0.05. Data are an average from at least three independent experiments. (B) Immunoblots depicting protein level of siRNA targets relative to scrRNA transfection from whole-cell lysates harvested 24 h posttransfection. Number above siRNA lane indicates percent knockdown of protein of interest relative to scrRNA transfection, normalized to GAPDH expression.