FIG 4.

Drug and vaccine candidates previously proposed in the literature within the 5th and 95th percentiles. It is striking that a large number of genes demonstrating high single-nucleotide variation (95th percentile) in our data set have been previously proposed in the literature as desirable drug candidates (gray bars) (22). Few of these drug targets have been previously selected due to their location (e.g., tatB) or their biological function (e.g., fadE33) (17, 22). In addition, three genes in the 95th percentile have been previously proposed as potential vaccine candidates (esxW, mpt53, apa) (black bars) (5, 21). In fact, esxW encodes an immunogenic protein, which is present in a current subunit vaccine (ID93/GLA-SE) (5). A smaller number of genes that are highly conserved in our data set (5th percentile) have been previously proposed as drug targets (gray bars). Genes in the 5th percentile, previously proposed as drug targets, have a median ratio of 0.109272 mutations/bp. Genes in the 95th percentile, previously proposed as drug targets, have a median ratio of 6.691533 mutations/bp. Biological functions and functional annotation of the genes are described in Tables 1 and 2.