Virulence of Argentinean CC30 MRSA major clades. (A) Schematic representation of predicted Ebh and SraP protein sequences and domains in the four clades. (B) Biofilm production by isolates of ARG-1 (N = 2), ARG-2 (N = 5), ARG-3 (N = 10), and ARG-4 (N = 45). Results are represented as final OD for each strain with median value (line) and interquartile range (whiskers) and were compared with Kruskal-Wallis test (P < 0.0001). The horizontal bars show significant differences between individual groups detected by Dunn’s multiple-comparison test. *, P < 0.05; ***, P < 0.001. NRS101 (S. epidermidis NRS101), strong biofilm producer; Δica (S. aureus Newman Δica), ica independent-biofilm producer strain. (C) Lipase activity on Baird-Parker agar plates. For each representative isolate of ARG-2, ARG-3, and ARG-4, 10 μl of 10-fold serial dilutions of a 0.5 McFarland suspension were plated.