TABLE 3.
Strains and plasmids
Strain or plasmid | Characteristics | Source or reference |
---|---|---|
Strains | ||
S. aureus | ||
AH1263 | USA300 LAC isolate cured of plasmid LAC-p03 | (47) |
RN4220 | Restriction-deficient transformation recipient | (46) |
RKC0654 | AH1263 pCK3 | This study |
RKC0660 | AH1263 pJB185 | This study |
E. coli | ||
DH5α | Cloning strain | Invitrogen |
Plasmids | ||
pCK3 | Paur-lacZ reporter plasmid; Ampr Cmr | (24) |
pJB138 | Promoterless codon-optimized lacZ reporter plasmid; Ampr Cmr | (24) |