TABLE 3.
Strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| S. aureus | ||
| AH1263 | USA300 LAC isolate cured of plasmid LAC-p03 | (47) |
| RN4220 | Restriction-deficient transformation recipient | (46) |
| RKC0654 | AH1263 pCK3 | This study |
| RKC0660 | AH1263 pJB185 | This study |
| E. coli | ||
| DH5α | Cloning strain | Invitrogen |
| Plasmids | ||
| pCK3 | Paur-lacZ reporter plasmid; Ampr Cmr | (24) |
| pJB138 | Promoterless codon-optimized lacZ reporter plasmid; Ampr Cmr | (24) |