TABLE 1.
Summary of proteins present in upper, middle, and lower molecular weight regions, identified by Western blot gel shift assays of whole-cell lysates of formaldehyde-cross-linked S. mutans detected with anti-YidC1, anti-YidC2, anti-Ffh, and anti-FtsY-specific antibodiesa
| Category | Upper region (reactive with anti-YidC2, anti-Ffh, and anti-FtsY antibodies) | Middle region (reactive with YidC1 and YidC2) | Lower region (reactive with anti-YidC1 antibodies) |
|---|---|---|---|
| Translation | 50S ribosomal protein L1 (SMU_1626)* | 50S ribosomal protein L1 (SMU_1626)* | Putative ribosomal protein S1; sequence-specific DNA-binding protein (SMU_1200)§ |
| 50S ribosomal protein L2 (SMU_2167)* | 50S ribosomal protein L2 (SMU_2167)* | Dimethyladenosine transferase, 16S rRNA methyltransferase (SMU_349) | |
| 50S ribosomal protein L7/L12 (SMU_960) | 50S ribosomal protein L5 (SMU_2015) | Putative tRNA pseudouridine synthase A (SMU_84) | |
| Putative ribosomal protein S1; sequence-specific DNA-binding protein (SMU_1200)§ | 50S ribosomal protein L18 (SMU_2010) | Translation elongation factor EF-Tu (SMU_714)§ | |
| 30S ribosomal protein S3 (SMU_2021)* | 30S ribosomal protein S2 (SMU_2032) | Conserved hypothetical protein, 16S rRNA methyltransferase (SMU_1659c) | |
| 30S ribosomal protein S5 (SMU_2009) | 30S ribosomal protein S3 (SMU_2021)* | ||
| 30S ribosomal protein S10 (SMU_2026c) | 30S ribosomal protein S13 (SMU_2003) | ||
| 30S ribosomal protein S11 (SMU_2002) | |||
| 30S ribosomal protein S18 (SMU_1858) | |||
| Translation elongation factor EF-Tu (SMU_714)§ | |||
| Translation elongation factor G (SMU_359) | |||
| Putative translation elongation factor TS (SMU_2031) | |||
| Translation initiation factor 2 (SMU_421) | |||
| Putative translation initiation factor IF3 (SMU_697) | |||
| Putative alanyl-tRNA synthetase (alanine-tRNA ligase) (SMU_650) | |||
| Translocation | Preprotein translocase subunit SecA (SMU_1838) | Putative preprotein translocase SecY protein (SMU_2006) | |
| Putative secreted protein, preprotein translocase subunit YajC (SMU_1787c) | |||
| Putative signal peptidase II (SMU_1661c) | |||
| Chaperones/proteases | Peptidyl-prolyl isomerase RopA (trigger factor) (SMU_91)§ | Peptidyl-prolyl isomerase RopA (trigger factor) (SMU_91)§ | |
| Heat shock protein. DnaK (HSP-70) (SMU_82) | Putative chaperonin GroEL (SMU_1954)§ | ||
| Putative chaperonin GroEL (SMU_1954)§ | |||
| Cell division/cell shape | Putative septation ring formation regulator (SMU_1276c) | Putative cell shape-determining protein MreC (SMU_20) | |
| Putative cell division protein DivIVA (SMU_557) | Putative cell division protein RodA (SMU_1279c) | ||
| Putative cell division protein FtsZ (SMU_552) | Integral membrane protein possibly involved in d-alanine export, d-alanyl-lipoteichoic acid biosynthesis protein DltB (SMU_1690) | ||
| Cell division protein FtsA (SMU_551) | Putative cell division protein FtsH (SMU_15)§ | ||
| Putative cell division protein FtsH (SMU_15)§ | Conserved hypothetical protein cell division protein FtsW (SMU_172) | ||
| DNA replication/repair | DNA-directed RNA polymerase, alpha subunit (SMU_2001) | Putative type II restriction endonuclease (SMU_506) | |
| DNA-dependent RNA polymerase, beta subunit (SMU_1990) | Putative site-specific DNA methyltransferase (SMU_504) | ||
| DNA-dependent RNA polymerase, beta' subunit (SMU_1989) | Putative endonuclease III (DNA repair) (SMU_1650) | ||
| Recombination protein RecA (SMU_2085) | Putative DNA polymerase III, delta subunit (SMU_1662) | ||
| Transcription | DNA-dependent RNA polymerase sigma subunit; major sigma factor (sigma 70/42) (SMU_822) | DNA-dependent RNA polymerase, beta' subunit (SMU_1989) | Conserved hypothetical protein, DNA-directed RNA polymerase subunit delta (SMU_1936c) |
| Putative tRNA isopentenylpyrophosphate transferase, tRNA dimethylallyltransferase (SMU_1477) | |||
| Putative substrates (proteins with one or more predicted transmembrane domains) | Putative septation ring formation regulator (SMU_1276c) | Hypothetical protein (SMU_591c)* | Conserved hypothetical protein peptide ABC transporter substrate-binding protein (SMU_1447c) |
| Putative ABC transporter, permease protein (SMU_1007) | Serine protease HtrA (SMU_2164) | Putative carbonic anhydrase precursor (SMU_1595) | |
| Putative PTS system, fructose-specific enzyme IIABC component (SMU_872) | Putative PTS system, mannose-specific component IID (SMU_1879) | Putative deacetylase (SMU_623c) | |
| Hypothetical protein (SMU_591c)* | Hemolysin (SMU_1693) | Conserved hypothetical protein, glycosyl transferase (SMU_834) | |
| Putative ABC transporter, ATP-binding protein ComA (SMU_286) | Hypothetical protein APQ13_07375 (ParE_toxin) (SMU_40) | Putative PTS system, glucose-specific IIABC component (SMU_2047)§ | |
| Conserved hypothetical protein, protease (SMU_235) | RgpH (SMU_832) | ||
| Putative cell division protein FtsH (SMU_15)§ | Putative amino acid ABC transporter, periplasmic amino acid-binding protein (SMU_933) | ||
| Putative PTS system, glucose-specific IIABC component (SMU_2047)§ | Conserved hypothetical protein, cyclic nucleotide-binding protein (SMU_1307c) | ||
| Putative PTS system, trehalose-specific IIABC component (SMU_2038) | Putative cell shape-determining protein MreC (SMU_20) | ||
| Putative glycosyltransferase, RgpI (SMU_833) | |||
| Conserved hypothetical protein (SMU_1477c) | |||
| Putative undecaprenyl-phosphate-UDP- MurNAc-pentapeptide transferase (SMU_456) | |||
| Putative cell division protein RodA (SMU_1279c) | |||
| Integral membrane protein possibly involved in d-alanine export, d-alanyl-lipoteichoic acid biosynthesis protein DltB (SMU_1690) | |||
| Putative transmembrane protein, permease OppC (SMU_257) | |||
| Conserved hypothetical protein (SMU_1111c) | |||
| Putative amino acid permease (SMU_1450) | |||
| Hypothetical protein (SMU_503c) | |||
| Hypothetical protein (SMU_1249c) | |||
| Putative preprotein translocase SecY protein (SMU_2006) | |||
| Putative sodium/amino acid (alanine) symporter (SMU_1175) | |||
| Putative glycosyl transferase N-acetylglucosaminyltransferase, RgpG (SMU_246) | |||
| Putative secreted protein, preprotein translocase subunit YajC (SMU_1787c) | |||
| Putative endolysin, N-acetylmuramoyl-l-alanine amidase (SMU_707c) | |||
| Putative manganese transporter (SMU_770c) | |||
| Putative serine/threonine protein kinase (SMU_484) | |||
| Putative cell division protein FtsH (SMU_15)§ |
|||
| Hypothetical protein (SMU_1161c) | |||
| Putative drug export protein; multidrug resistance protein, XRE family transcriptional regulator (SMU_745) | |||
| Putative ABC transporter, periplasmic ferrichrome-binding protein (SMU_998) | |||
| Cell wall-associated protein precursor WapA (SMU_2159) | |||
a
Proteins present in both upper and middle molecular weight gel slices (*) and proteins detected in regions reactive with both anti-YidC1 and anti-YidC2 antibodies (§) are indicated. PTS, phosphotransferase system.