TABLE 3.
Functional categories of proteins captured with anti-YidC2 coupled Dynabeads from S. mutans whole-cell lysates but absent from ΔyidC2 mutant strain samples
| Functional category and proteina |
|---|
| Translation |
| 50S ribosomal protein L14 (SMU_2017) |
| 50S ribosomal protein L31 type B (SMU_1298) |
| 50S ribosomal protein L16 (SMU_2020) |
| tRNA uridine 5-carboxymethylaminomethyl modification protein (SMU_2141) |
| Translocation |
| Preprotein translocase subunit SecA (SMU_1838) |
| Preprotein translocase subunit YajC (SMU_1787c) |
| DNA replication/repair |
| DNA-directed RNA polymerase subunit beta' (SMU_1989) |
| DNA repair protein RadA (SMU_327) |
| Deoxyribonuclease HsdR |
| DNA topoisomerase I (SMU_1002) |
| DNA topoisomerase IV subunit B (SMU_1277) |
| Chaperones/proteases |
| Metalloprotease RseP* (SMU_1784c) |
| ATP-dependent Clp protease ATP-binding subunit (SMU_956) |
| Cell wall/cell shape/cell division |
| Penicillin-binding protein Pbp2b (SMU_597) |
| Peptidoglycan branched peptide synthesis protein MurM* (SMU_716) |
| Septation ring formation regulator EzrA (SMU_1276c) |
| d-Alanyl-lipoteichoic acid biosynthesis protein DltD (SMU_1688) |
| Rod shape-determining protein RodA (SMU_1279c) |
| Cell division protein SepF (SMU_554) |
| Putative substrates (with one or more predicted TM domains) |
| Penicillin-binding protein (SMU_597) |
| Metalloprotease RseP* (SMU_1784c) |
| Septation ring formation regulator EzrA (SMU_1276c) |
| Cytoplasmic membrane protein, LemA (SMU_1930) |
| d-Alanyl-lipoteichoic acid biosynthesis protein DltD (SMU_1688) |
| Serine/threonine protein kinase (SMU_484) |
| Preprotein translocase subunit YajC (SMU_1787c) |
| PTS mannose family transporter subunit IID (SMU_1879) |
| Hypothetical protein APQ13_00045 (SMU_1719c) |
| Acyltransferase (SMU_67) |
| ABC transporter permease (SMU_396) |
| Hypothetical protein APQ13_06235 (SMU_333) |
| Hypothetical protein APQ13_07285 (SMU_66) |
| PTS mannose transporter subunit IIC (SMU_1878) |
| Hemolysin (SMU_1693) |
| Phosphatidate cytidylyltransferase (SMU_1785) |
| Murein hydrolase transporter LrgA (SMU_575c) |
| Glycosidase |
| Hypothetical protein APQ13_09110 (SMU_1856c) |
| Rod shape-determining protein RodA (SMU_1279c) |
| Hypothetical protein APQ13_07320 |
| PAS domain-containing sensor histidine kinase (SMU_1516) |
| Histidine kinase (SMU_1145c) |
a
Proteins present in both formaldehyde-cross-linked and non-cross-linked samples are indicated by an asterisk.