FIG 7.
Single-dose intratracheal Sph3h prophylaxis is associated with early pulmonary neutrophil recruitment. Leukopenic mice were intratracheally infected with 5 × 103 wild-type (WT) A. fumigatus conidia coadministered with or without 500 μg Sph3h. Pulmonary leukocyte populations were quantified by flow cytometry at 1 day and 2 days after Sph3h prophylaxis. Bars represent the means ± standard errors from 3 independent experiments for uninfected buffer-treated (UI + buffer), wild-type-infected buffer-treated (WT + buffer), and wild-type-infected Sph3h-treated (WT + Sph3h), and 2 independent experiments for uninfected Sph3h-treated (UI + Sph3h) group, with ≥6 mice per group. A significant increase in leukocyte populations is indicated (*, P < 0.0001) relative to the uninfected buffer-treated (UI + buffer) group at 1 day and 2 days as determined by two-way ANOVA with Tukey’s multiple-comparison test.