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Analysis of the binding properties of acinetobactins for various metal ions. (A to I) The UV-visible absorption spectra of Oxa (1) and Isox (1) after 24 h of incubation in 100 mM Tris·HCl buffer (pH 7) at room temperature containing (A) no additive, (B) FeCl3, (C) MgSO4, (D) CaCl2, (E) ZnSO4, (F) CuCl2, (G) CoCl2, (H) MnSO4, and (I) NiCl2. The wavelengths of distinctive peaks are labeled.
