FIG 6.

Growth-promoting activity assays in the presence of human metal sequestering proteins. Growth of A. baumannii ΔbasD in the presence of different concentrations of apo-Oxa (1, red bar) or apo-Isox (2, blue bar) under iron-deficient conditions created by supplementation of apo-transferrin (1 mg/ml), apo-lactoferrin (2 mg/ml), or apo-calprotectin (20 μM) (Fig. 5). After incubation at 37°C for 12 h, the absorbance at 600 nm was recorded using a microplate reader. The time course growth curves over 24 h can be found in Fig. S6. The error bars represent the standard deviations of independent triplicate experiments. Statistical significance was assessed by two-way ANOVA tests (ns, not significant; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).