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The myosin light chain 1 is not required for filamentous growth or Spitzenkörper formation. Indicated strains (control; mlc1Δ/MLC1 [PY5018] and mlc1 [mlc1Δ/mlc1Δ, PY5451]) expressing Scarlet-Sec4 were incubated and imaged as described in the legend to Fig. 1A, and sum projections are shown with the second image in each panel 1 h after the first image.
