FIG 5.
Synthetic interaction of Mlc1 with either Sec4 or prenylated GFP perturbs filamentous growth. (A) Schematic showing plasma membrane targeting of Mlc1 or stabilization with Sec4. The different domains and fusion proteins are indicated in the box. (B) Altering the stability or distribution of Mlc1 at the Spitzenkörper perturbs filamentous growth. Wild-type cells expressing Mlc1-iRFP and GFP·Sec4 (Control; PY4809), Mlc1-iRFP-GNB (iRFP stands for near-infrared fluorescent protein and GNB for GFP nanobody) and GFP-Sec4 (Mlc1·Sec4-stablilized; PY5405) or Mlc1-iRFP-GNB and GFP-CtRac1 (Mlc1·GFP prenylated; PY5409) were imaged as described in the legend to Fig. 2A. Note that in the absence of Mlc1-iRFP-GNB, GFP-CtRac1 (Rac1 carboxy terminus) is observed uniformly on the plasma membrane (62, 63) (Fig. 7A).