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. 2021 Mar 21;11(10):3322–3334. doi: 10.1016/j.apsb.2021.03.029

Figure 6.

Figure 6

Optimization of baccatin III production by fed-batch fermentation. The fermentation was carried out in a 3-L bioreactor with 2.0 g/L 10-DAB and different concentrations of acetic acid supplemented at the beginning of production stage (a–f). (A) 30 mmol/L acetic acid (initial pH 6.0). (B) 50 mmol/L acetic acid (initial pH 5.5). (C) 65 mmol/L acetic acid (initial pH 5.0). (D) DBAT expression under different initial acetic acid concentrations. (E) Baccatin III titers and 10-DAB conversion rates under different initial acetic acid (HAc) concentrations at 55 h of cultivation. (F) Baccatin III titers and 10-DAB conversion rates under different maintaining pH at 55 h of cultivation. (G) Baccatin III titers and 10-DAB conversion rates under different 10-DAB concentrations and the same maintaining pH (5.8) at 55 h of cultivation. The data represent the means ± SD, n = 3.