FIG 2.

SdjA selectively rescues the yeast toxicity of members of the SidE family. A plasmid that expresses SdjA from the glyceraldehyde 3-phosphate dehydrogenase promoter was introduced into yeast strains harboring SidE, SdeA, SdeB, or SdeC expressed from a galactose-inducible promoter. Similar amounts of yeast cells were diluted and spotted onto medium supplemented with glucose or galactose. Images of the plates were acquired 4 days after incubation at 30°C (top). The expression of sdjA in these yeast strains was detected after galactose induction. The metabolic enzyme 3-phosphoglycerate kinase (PGK) was probed as a loading control (bottom).