FIG 3.
Impacts of cfp disruption on radial growth and stress response of B. bassiana. (A) Diameters of fungal colonies grown at 25°C for 8 days on rich SDAY, minimal CDA, and CDAs amended with different carbon (trehalose, glucose, glycerol, or sodium acetate [NaAc]) or nitrogen sources and deleted carbon source (dC) or nitrogen source (dN). (B and C) Images (scale, 2 cm) and relative growth inhibition (RGI) percentages of fungal colonies grown at 25°C for 8 days on CDA containing indicated concentrations of chemical stressors (SBT, sorbitol; CGR, Congo red; CFW, calcofluor white; MND, menadione). All colonies were initiated by spotting 1-μl aliquots of a 106-conidia/ml suspension. (D) Total SOD and catalase (CAT) activities quantified in the protein extracts of 3-day-old SDAY cultures. *, P < 0.05 in Tukey’s HSD test. Error bars, SDs of the means from three independent replicates.