TABLE 2.

Results of the diagnostic multiplex PCR^a,b

Host	Target gene	No. of PCR positive samples for:		P value
		Normal (n = 53)	Diarrhea (n = 53)
Viruses
Group a rotavirus	dsRNA segment 6	3	1	0.1574
Group a rotavirus	VP7	0	0
Group B rotavirus	VP7	0	0
Group C rotavirus	VP6	1	4	0.0870
Bovine coronavirus	N	1	1	0.4947
Bovine coronavirus	S	0	0
Bovine torovirus	M	0	0
Bovine torovirus	N	0	0
Bovine norovirus	RdRp	2	1	0.2837
Bovine enteric Nebraska-like calicivirus	RdRpMCP	3	1	0.1574
Bovine nebovirus	Capsid	0	0
Bovine viral diarrhea virus	BstEII	0	0
Bovine viral diarrhea virus	BVDV1 genotype	3	1	0.1574
Bovine viral diarrhea virus	Polyprotein	0	0
Bacteria
Clostridium perfringens	16S rRNA gene	16	24	0.0557
Clostridium perfringens	Alpha-toxin genes	5	3	0.2342
Salmonella enterica	sefb	0	0
Salmonella enterica Typhimurium	rfbJ	0	0
Enterotoxigenic Escherichia coli	K99	0	0
Shigatoxigenic Escherichia coli	stx1	15	10	0.1283
Shigatoxigenic Escherichia coli	stx2	13	18	0.1449
Shigatoxigenic Escherichia coli	eaeA	10	8	0.3050
Enterohemorrhagic Escherichia coli	hlyA	18	18	0.4985

^aAbbreviations: dsRNA segment 6, double-stranded RNA genome segment 6; VP, viral protein; N, nucleocapsid protein; S, S glycoprotein; M, membrane protein; RdRp, RNA-dependent RNA polymerase; MCP, major capsid protein; BstEII, restriction enzyme BstEII; sefB, chaperone protein SefB coding gene; rfbJ, CDP-abequose synthase coding gene; K99, K99 region 1 gene; stx1, Shiga toxin type 1; stx2, Shiga toxin type 2; eaeA, enterohemorrhagic E. coli O157:H7-specific intimin; hlyA, plasmid-encoded enterohemolysin.

^bThe data were analyzed using the nonparametric Mann-Whitney U test (one-tailed).