FIG 1.
PolyFermS setup of immobilized and planktonic adaptation and further experiments in in vitro human adult gut microbiota. (A) Immobilized L. plantarum NZ3400 was added to immobilized fecal gut microbiota in IR1 from donor 1 during a single-stage fermentation. (B) Immobilized fecal microbiota of donors 2 to 4 were cultivated in the inoculum reactor (IR), which was used to inoculate second-stage reactors (TRs) that were supplemented with L. plantarum after stabilization. NZ3400, reference strain; NZ3400B, new stock from single-colony isolate of NZ3400; IA10, IA01, PA1.2_01, and PA2_06, recovered L. plantarum mutants; L. plantarum ΔLP_RS14990, LP_RS14990 gene deletion strain; FM, inflow MacFarlane medium; FO, reactor outflow; S/W, sampling/waste.