FIG 4.

Conserved pangenome members of Sinorhizobium are actively transcribed in nodules. (A) Hierarchical divisions of core/accessory subsets for the five test Sinorhizobium strains. Genus core = subset I; core genes shared by five strains = subset I + subset II; genes shared by two to four strains = subset I + subset II + subset III; strain-specific genes = subset IV. (B) Core genes localized on the same (left) or different (right) replicons in the multipartite genomes of five test strains. Lines in green, red, and yellow represent homologs on the chromosome (SF4c, SF2c, SF8c, SS1c, and SJ4c), chromid (SF4b, SF2b, SF8b, SS1b, and SJ4b), and symbiosis plasmid (SF4a, SF2a, SF8a, SS1a, and SJ4a), respectively. Replicons are shown in a linearized way with the length of fragments corresponding to sizes in the Mb scale. (C) Replicon-dependent transcriptional levels of pangenome subsets defined in panel A. C.I., confidence interval.