Fig. 1.

Effect of PLM on cytotoxicity, NO, PGE2_, TNF-α, IL-1β, and IL-6 productions in LPS-stimulated RAW264.7 cells. Cells were pretreated with PLM (0, 50, 100, 200, 500 μg/mL) for 2 h and then with LPS (0.5 μg/mL) for 24 h. A Cell viability was measured with an MTT assay. B Collected supernatants were reacted with Griess reagent, and the absorbance was measured at 540 nm. The supernatants were collected and subjected to ELISA for (C) PGE2, D TNF-α, E IL-1β, and F IL-6. The values are expressed as the mean ± SEM (n = 5). ^###p < 0.001 vs. control cells, *p < 0.05; **p < 0.01, ***p < 0.001 vs. LPS-treated cells