Fig. 4.

Effects of PLM on immune cells subtype in the thymus. Mice were pretreated with KRG (100 mg/mL) or PLM (100, and 200 mg/mL) orally for 5 weeks. Moreover, all mice except normal mice were stimulated with 10% PP treated 3 days before the sacrifice and 2% starch treated 2 days before the sacrifice. The thymus was collected and measured through fluorescent antibody cell sorting (FACS). A CD4⁺CD25⁺ absolute numbers, B CD4⁺& CD8⁺ absolute numbers, and C CD4⁺CD69⁺ absolute numbers. The values are expressed as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control mice