Table 1.
In vitro 13C isotope effects of aldolase and p-GAPDH (Canellas and Cleland, 1991; Gleixner and Schmidt, 1997)
| Aldolase | p-GAPDH | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| FBP | DHAP | + | GAP | GAP | 1,3BPGA | ||||
| C-3 | C-4 | C-3 | C-4 | C-4 | C-4 | ||||
| → | 1.0159 | 0.9968 | → | 1.0097 | |||||
| 0.9964 | 0.9951 | ↔ | 1.0036 | 1.0049 | 1.0078 | ↔ | 0.9922 | ||
| 1.0123 | 0.9919 | ← | 1.0176 | ← |
→ Kinetic isotope effect, ↔ equilibrium isotope effect. Carbon numbering corresponds to related carbon positions in glucose. Reported values are in italics. Calculated values are not italicized. Effects of p-GAPDH refer to free GAP, not hydrated GAP.