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. 2021 Oct 12;12:686769. doi: 10.3389/fimmu.2021.686769

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Copyright © 2021 Olmsted-Davis, Mejia, Salisbury, Gugala and Davis

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single-cell transcriptome analysis of ADRβ3+ cells isolated from regions of active bone formation. Analysis of single-cell RNAseq transcriptomes using the Seurat (v3) algorithm. This analysis was done several times with similar results. Transcriptomes associated with the ADRβ3+ cells isolated from (A) HO and (B) fracture callus. DotPlots of macrophage transcript expression using the Seurat algorithm in (C) HO and (D) fracture callus. DotPlot depiction of osteoclast-associated transcript expression in (E) HO and (F) fracture repair.