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. 2021 Oct 12;12:686769. doi: 10.3389/fimmu.2021.686769

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Copyright © 2021 Olmsted-Davis, Mejia, Salisbury, Gugala and Davis

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Co-embedding of the Seurat from HO with that from fracture callus. In order to more accurately assess common features between the MH and FC clusters they were co-embedded by first processing the gene activity matrix in order to find anchors between cells in the MH and FC datasets. These anchors were then used to transfer the data by the standard workflow in Seurat to plot the co-embedded dataset. (B) The percentage of the top 50 transcripts of each MH cluster present in FC5 are indicated. Although cluster MH7 has no transcripts in FC5, it has 62% of its transcripts present in FC9.