FIG. 5.

Quantitation of the effect of His₆-p27 on the elution profile of cyclin E complexes. Lysate from exponentially growing cells was incubated with the indicated concentrations of His₆-p27 (▴) as described for Fig. 3 and together with lysate from ORG 2058-treated cells (●) was fractionated by gel filtration/SDS-PAGE and Western blotted as described for Fig. 1. Densitometric analysis of the Western blot data is displayed for each treatment compared to an untreated control sample without added His₆-p27 (○). The relative cyclin E kinase activity obtained from an aliquot of each incubation is also displayed.