FIG. 7.

Association of Cdk4 and INK4 inhibitors. (A) Lysates were prepared as described for Fig. 1, and p15^INK4b and p18^INK4c immunoprecipitates were Western blotted with the cognate antibodies and with antibodies to Cdk4 and Cdk6 as indicated. (B) Lysates were prepared from T-47D cells treated for 6, 12, 18, or 24 h with ORG 2058 or ethanol vehicle; Cdk4 and p18^INK4c complexes were immunoprecipitated in parallel and Western blotted using the cognate antibodies.