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. 2021 Oct 26;41(11):e00122-21. doi: 10.1128/MCB.00122-21

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FIG 1 — Maximized z-stack confocal images of the HttQ103 and HttQ103P fragments expressed under different conditions. (a and A) HttQ103 and HttQ103P fragments in [RNQ⁺] [PSI⁺] yeast following growth overnight in galactose medium. (b and B) HttQ103 and HttQ103P fragments in yeast cured of prions by guanidine following growth overnight in galactose medium. (c and C) HttQ103 and HttQ103P fragments in [RNQ⁺] [PSI⁺] yeast expressed for 9 h (∼3 generations) in galactose medium. (d and D) HttQ103 and HttQ103P fragments in [RNQ⁺] [PSI⁺] yeast expressed for 9 h in galactose and guanidine medium. (e and E) HttQ103 and HttQ103P fragments in yeast grown for 6 h with galactose and guanidine, spun, and resuspended in galactose medium for 3 h. (f and F) HttQ103 and HttQ103P fragments in [RNQ⁺] [PSI⁺] yeast grown overnight at a relatively high density (starting OD of ∼0.4) in galactose medium with guanidine. The insets are an enlargement of the profile drawn through an aggregate. Images of HttQ103 and HttQ103P are shown in panels a to f and A to F, respectively.