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Maximized z-stack images of HttQ103 fragments expressed in yeast cured of prions by guanidine as a function of time in galactose medium. (A to D) Yeast cells were grown in galactose medium for 1 to 4 days. (E and F) A colony from a galactose plate was resuspended in galactose medium and grown overnight in galactose medium (E), followed by growing cells overnight in 5 mM guanidine (F).
