FIG 6.

Dot blot and Western blot assays of HttQ103. (A) Dot blot of HttQ103 from yeast lysates that were either not treated with SDS buffer or boiled in 2% SDS. Lane 1, lysate prepared from [PRION⁺] yeast cells expressing HttQ103 from the GAL1 promoter, which was integrated into the chromosome; lane 2, lysate prepared from [prion⁻] yeast cells expressing HttQ103 from the GAL1 promoter integrated into the chromosome that were cultured on galactose plates and then grown on galactose medium; lane 3, lysate prepared from [PRION⁺] yeast cells expressing HttQ103 from the GAL1 promoter on a 2μm plasmid. The same concentration of total protein was added to each well. (B) Western blotting of HttQ103 from yeast lysates grown under different conditions. Lanes 1 and 3, total lysates that were boiled in SDS buffer; lanes 2 and 4, lysates treated with formic acid before boiling in SDS buffer. Lanes 1 and 2 are lysates prepared from [PRION⁺] yeast cells expressing HttQ103 from the GAL1 promoter integrated into the chromosome. Lanes 3 and 4 are lysates prepared from [prion⁻] yeast cells expressing HttQ103 from the GAL1 promoter integrated into the chromosome that were cultured on galactose plates and then grown on galactose medium. [PRION⁺] yeast contains both the [RNQ⁺] and [PSI⁺] prions. [prion⁻] yeast cells are cured of all prions.