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Effect of Hsp104 on the aggregation of HttpolyQ fragmentsa
| Construct | Condition(s) | % of cells with foci | % of cells with >10 foci per cell |
|---|---|---|---|
| Htt-Q103 | 9 h Gal | >95 | 90 |
| Htt-Q103 | 9 h Gal+Gdn | >70 | 5 |
| Htt-Q103 | 6 h Gal+Gdn, 3 h Gal | >95 | 70 |
| Htt-Q103P | 9 h Gal | >95 | 80 |
| Htt-Q103P | 9 h Gal+Gdn | >78 | 3 |
| Htt-Q103P | 6 h Gal+Gdn, 3 h Gal | >80 | 12 |
Experiments were performed in triplicates under each condition. A total of 350 cells were counted for each sample. Gal, galactose; Gdn, guanidine.
