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. 2021 Oct 12;9:675356. doi: 10.3389/fcell.2021.675356

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Copyright © 2021 Xu, Xiao, Fan, Ruan, Cheng, Tian, Chen, Chen and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RFWD3 knockdown inhibited colorectal cancer development in vitro. (A) The knockdown efficiencies of shRFWD3-1, shRFWD3-2, and shRFWD3-3 were detected by qRT-PCR. (B) The cell proliferation rate was evaluated in colorectal cancer cell lines after infection by Celigo cell counting assay. (C,D) The migration rate of cells was detected in colorectal cancer cell lines after infection by transwell assay (C) and wound-healing assay (D). Magnification times: 200×. (E,F) The effects of RFWD3 knockdown on cell apoptosis (E) and cell cycle (F) were examined by flow cytometry. (G) The levels of phosphorylation-related proteins in RKO cells infected with shCtrl and shRFWD3 were measured using a human phosphorylation array. Protein expression was presented in gray value. (H) The expression of CCND1, CDK6, Bcl-2, IGFBP3, and CASP3 was detected by western blot. Results were presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.