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. 2021 Oct 12;9:675356. doi: 10.3389/fcell.2021.675356

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Copyright © 2021 Xu, Xiao, Fan, Ruan, Cheng, Tian, Chen, Chen and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RFWD3 knockdown inhibited colorectal cancer tumor growth in vivo. (A) A nude mice model of RFWD3 knockdown was constructed. (B) The fluorescence intensity was obtained through injection of D-Luciferase before sacrificing the mice. (C) L and W of tumors were recorded to obtain tumor volume (L represent longest dimension and W means dimension perpendicular to length, and tumor volume was calculated as π/6 × L × W2). (D) The weight of tumors was measured after sacrificing mice. (E) The photograph of tumors was taken after removing tumors. (F) The level of Ki-67 was detected by IHC in tumor sections from mice. Results were presented as mean ± SD. ** P < 0.01, *** P < 0.001.