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. 2021 Oct 12;9:675356. doi: 10.3389/fcell.2021.675356

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Copyright © 2021 Xu, Xiao, Fan, Ruan, Cheng, Tian, Chen, Chen and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exploration and verification of underlying mechanism of RFWD3 regulating colorectal cancer. (A) The heatmap of DEGs was identified by Affymetrix Human Gene Chip Prime View analysis in HCT 116 cells infected with shCtrl (n = 3) or shRFWD3 (n = 3). (B,C) The expression of several most significantly DEGs was determined by qRT-PCR (B) and western blot (C) in HCT 116 cells following infection. (D) Co-IP assay was used to verify whether there was protein interaction between RFWD3 and E2F1. (E) The dual-luciferase assay was conducted to explore whether RFWD3 regulated BIRC5 promoter activity. (F) ChIP-qPCR assay was conducted to confirm that E2F1 bound to the CDK1 promoter. The data are expressed as mean ± SD. * P < 0.05, *** P < 0.001.