FIG 1.
Generation of myeloid lineage-specific HO-1-knockout mice. (A) The HO-1 gene is floxed by two loxP sites, and the DsRed gene has been inserted. i.p., intraperitoneal. (B) Thioglycolate-induced induction of peritoneal macrophages isolated from HO-1+/+, HO-1flox/flox, and HO-1flox/flox::LysMCre mice. The cells were treated with 100 μM DEM, 10 nM CDDO-Im, or DMSO (vehicle). (C and D) Relative mRNA level of HO-1 after 3 h of treatment with DEM, CDDO-Im, or DMSO (vehicle). (E and F) Relative mRNA level of NQO1 after 3 h of treatment with DEM, CDDO-Im, or DMSO (vehicle). ***, P < 0.001.