FIG 2.
HO-1–DsRed expression in macrophages by Cre-mediated recombination of the HO-1 flox allele. Thioglycolate-induced peritoneal macrophages were isolated from HO-1+/+, HO-1flox/flox, and HO-1flox/flox::LysMCre mice and treated with 100 μM DEM or DMSO (vehicle). (A) HO-1, NRF2, and tubulin protein levels in the macrophages after 12 h of treatment were examined by Western blot analysis. Open and closed arrowheads indicate HO-1-DsRed and endogenous HO-1, respectively. (B) Representative DsRed fluorescence microscopy images of peritoneal macrophages after 24 h of treatment with 100 μM DEM or DMSO (vehicle). Scale bars, 50 μm.