FIG 3.

IVIS analyses show in vivo HO-1–DsRed induction by CDDO-Im and hemin in HO-1^DsRed/+ mice. (A) The DsRed gene was knocked-in the HO-1 gene locus. (B) HO-1^+/+ and HO-1^DsRed/+ mice were treated with CDDO-Im, hemin, or vehicle 72 h before IVIS analysis. (C) Representative whole-body DsRed fluorescence images of HO-1^+/+ and HO-1^DsRed/+ mice orally treated with 30 μmol/kg of BW of CDDO-Im or vehicle. (D) Representative whole-body DsRed fluorescence images of HO-1^+/+ and HO-1^DsRed/+ mice peritoneally injected with 30 mg/kg hemin. (E and F) Representative DsRed fluorescence images of the organs isolated ex vivo from HO-1^+/+ and HO-1^DsRed/+ mice after 72 h of treatment with CDDO-Im (E) and hemin (F).