FIG 7.
NRF2-independent HO-1–DsRed induction by hemin. Thioglycolate-induced peritoneal macrophages were isolated from HO-1DsRed/+ and Nrf2−/−::HO-1DsRed/+ mice. (A and B) Representative images (A) and relative DsRed fluorescence intensity (B) in macrophages after 48 h of treatment with hemin. Note that Nrf2 knockout did not affect the induction of HO-1 gene expression by hemin. Scale bars, 200 μm. (C) Protein levels of HO-1 and HO-1–DsRed in macrophages after 3 h of treatment with hemin. Open and closed arrowheads indicate HO-1-DsRed and endogenous HO-1, respectively. (D and E) Representative images (D) and relative DsRed fluorescence intensity (E) in macrophages after 48 h of treatment with CBD. Scale bars, 200 μm.