TABLE 2.
Sequences of primers and probes used for RT-PCRa
| Assay and gene | Sequence |
|---|---|
| RT-PCR (TaqMan) | |
| NQO1 | F: AGCTGGAAGCTGCAGACCTG |
| R: CCTTTCAGAATGGCTGGCA | |
| P: ATTTCAGTTCCCATTGCAGTGGTTTGGG | |
| HPRT | F: CTGGTGAAAAGGAACCTCTCG |
| R: TGAAGTACTCATTATAGTCAAGGG | |
| P: ATCCAACAAAGTCTGGCCTGTATCCAAC | |
| RT-PCR (SYBR) | |
| HO-1 CT | F: CACGCATATACCCGCTACCT |
| R: CCAGAGTGTTCATTCGAGCA | |
| SPIC | F: TGGTAGACAGCATTTACCCTCA |
| R: CAGGCCACCATCCTGTTCTG | |
| DsRed | F: CCCCGTAATGCAGAAGAAGACT |
| R: GATTGACTTGAACTCCACCAGGT |
a
F, forward primer; R, reverse primer; P, probe.