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. 2021 Sep 14;6(5):e00879-21. doi: 10.1128/mSystems.00879-21

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Copyright © 2021 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Detection of ubiquitination by ubiquitylome analysis and Western blot analysis. (A) Statistical result of ubiquitylated proteins and sites in ubiquitylome data. (B) Heat map analysis of quantified ubiquitylation sites. (C) Equalization of total amounts of proteins between different conidium sample by running short SDS-PAGE (0.3 cm). (D) SDS-PAGE analysis of total proteins. (E) Detection of ubiquitination by anti-ubiquitin antibody in Western blot. AO-Ck, fresh conidia; AO-G24, germinated conidia; AO-Am, fungistatic conidia.