MIC, growth curve, survival, and gene mutation of AP-RCLIN, AP-RCLIN-EVO, and AP-RLAB-EVO. (A) MIC of the three bacterial strains to SCF. (B) Growth curve of the three bacterial strains in LB medium. (C) Survival of the three bacterial strains in the presence of the indicated concentration of SCF. (D) Mutations detected in whole-genome sequencing of AP-RCLIN-EVO and AP-RLAB-EVO compared to AP-RCLIN. Results in panels B and C are displayed as means ± SEM, and significant differences as determined by two-tailed Student’s t test are identified (*, P < 0.05; **, P < 0.01).