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. 2021 Sep 21;6(5):e00732-21. doi: 10.1128/mSystems.00732-21

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Copyright © 2021 Kuang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Effect of fumarate-based NO biosynthesis on SCF-mediated killing. (A) qRT-PCR for expression of genes encoding the P cycle and nitrite-dependent NO in the presence of fumarate. (B) Outline for changes based on data in panel A. Blue, genes detected; gray, genes undetected. (C and D) Percent survival of the three strains at the indicated concentrations of fumarate (C) and SCF (D). (E) NADH in the presence of fumarate. (F) Nitrite in the presence of fumarate. (G and H) Percent survival of the three strains in the indicated concentrations of NADH (G) and SCF (H). Results in panels A and C to G are displayed as means ± SEM, and significant differences as determined by two-tailed Student’s t test are identified (*, P < 0.05; **, P < 0.01).