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. Author manuscript; available in PMC: 2023 Sep 26.
Published in final edited form as: J Med Chem. 2021 Oct 19;65(4):2940–2955. doi: 10.1021/acs.jmedchem.1c01307

Figure 6. Association and dissociation rates and binding cooperativity.

Figure 6.

SPR was used to measure (A) association rates and (B) dissociation rates for PLpro inhibitors: GRL0617 (1), ZN-2-184 (5), ZN-3-80 (65), XR8-24 (73), XR8-23 (72), XR8-89 (94). (C) Binding affinity (KD determined by SPR) was used to demonstrate potential cooperativity by engaging multiple weak interactions across multiple binding sites.