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. 2021 Oct 25;9(10):e002699. doi: 10.1136/jitc-2021-002699

Figure 1.

Figure 1

PD-L2 is N-glycosylated and upregulated in patients with cetux-resistant HNSCC. (A) T-distributed stochastic neighbor embedding (t-SNE) plot of cancer cells in two patients with HNSCC before and after cetux treatment. (B) t-SNE plot showing three different groups identified based on density-based clustering. (C) Violin plot depicting PD-L2 expression levels in the three different groups determined using single-cell RNA sequencing. ***p<0.001; ****p<0.0001. (D) KEGG pathway enrichment analysis revealed that cetux refractoriness is correlated with the ‘proteoglycans in cancer’ pathway. (E) Immunohistochemical staining of PD-L2 in five cetux-resistant samples and nine cetux-sensitive HNSCC tissues. Scale bar, 50 µm. One-way analysis of variance (**p<0.01). (F) Protein expression of PD-L2 in clinical HNSCC samples obtained at the Tianjin Medical University Cancer Institute and Hospital. HNSCC, head and neck squamous cell carcinoma; KEGG, Kyoto Encyclopedia of Genes and Genomes; PD-L2, programmed death ligand 2; t-SNE, t-distributed stochastic neighbor embedding.