FIG. 7.

Ear-2 suppresses basal and T3-induced responses of the human S14 promoter. The data are from a representative experiment that was repeated three times. Each bar is the mean + standard deviation of three plates with the indicated treatment. Bars 1, 3, 5, and 7 were hepatocytes maintained at 5.5 mM glucose and no T3; bars 2, 4, 6, and 8 were cells maintained at 27.5 mM glucose and 500 nM T3. As indicated below the bars, TRβ1 and Ear-2 were cotransfected as indicated in Materials and Methods. At each condition, the addition of T3 led to a significant (P, <0.05) increase in luciferase activity. At each condition, the cotransfection of Ear-2 led to a significant (P, <0.05) decrease in luciferase activity.