FIG. 9.

Reversal of the Ear-2 repression of the T3-dependent transcriptional activity of TRβ1 by SRC-1 in CV1 cells. CV1 cells were cotransfected with the CAT reporter plasmid (pTK28m; 0.2 μg), the TRβ1 expression plasmid (pCDM-TRβ1; 0.2 μg), the β-galactosidase expression vector (pCH110; 0.2 μg), and the SRC-1 expression vector (pCR3.1 hSRC-1A; 1 μg) in the absence (lanes 1 to 4, 9 to 12, and 17 to 20) or the presence of increasing concentrations of pCDM-Ear-2 (lanes 6 to 9, 0.025 μg; lanes 14 to 17, 0.05 μg; lanes 22 to 25, 0.1 μg) as described in Materials and Methods. The total plasmids transfected were normalized to 3 μg by using the vector control plasmid, pCR3.1. The final ratios of Ear-2 to TRβ1 are indicated. The data were from three independent experiments, each with duplicates (mean ± standard deviation; n = 3).