Fig 1. Card19lxcn BMDMs are deficient for caspase-dependent cell death.
Primary C57BL/6J (B6) or Card19lxcn BMDMs were infected with Salmonella enterica serovar Typhimurium (S. Tm) or Y. pseudotuberculosis (Yptb), or treated with LPS+ATP or staurosporine (Sts), as indicated, and the kinetics of cell death was assayed by release of lactate dehydrogenase (LDH) (A, B, E, F) or propidium iodide (PI) uptake (C, D, G, H). Each figure is representative of three or more independent experiments. LDH release was assayed at specific times post-infection. PI uptake was measured over a two or ten-hour timecourse with fluorometric measurements taken at 2.5 minute (C, D) or 10 minute (G, H) intervals. Mean ± SEM of triplicate wells is displayed. Each panel is representative of three or more independent experiments. *** p < 0.001, ** p < 0.01, * p < 0.05. n.s. not significant, analyzed by 2-way ANOVA with Bonferroni multiple comparisons post-test.