Fig 3. Peritoneal macrophages from Card19^lxcn mice have reduced levels of cell death.

(A) B6 and Card19^lxcn mice were injected with 4% aged thioglycolate or PBS. 48 hrs later, PECS were harvested, RBC lysed, counted and plated in triplicate overnight at 37°C. PBS PECS were pooled prior to plating. Cells were washed with PBS to remove non-adherent cells and infected with Yptb or S. Tm (MOI 10) or treated with staurosporine (10 uM). Cytotoxicity was measured by LDH release at indicated time points. *** p < 0.001, ** p < 0.01, * p < 0.05. n.s. not significant. 2-way ANOVA with Bonferroni multiple comparisons post-test. Graphs are representative of two independent experiments with 3–6 mice per group, per genotype. (B, C) PBS: mean ± SEM for technical replicates of 3 pooled B6 mice. Thioglycolate: means of technical replicates for 3 B6 mice ± SEM. Cells were infected with (B) S. Tm or (C) Yptb. (D, E) PBS: mean ± SEM for technical replicates of 4–6 pooled B6 and Card19^lxcn mice + SEM. Thioglycolate: means of technical replicates for 4 B6 and Card19^lxcn mice ± SEM. Cells were infected with (B) S. Tm or (C) Yptb.