Fig 8. NINJ1 mediates anti-Yersinia host defense.

(A) Survival of B6 and Card19^lxcn mice following oral infection 10⁸ CFUs of strain IP2777 Yptb. Data are pooled from two independent experiments that each gave similar results. Log-rank (Mantel-Cox) Survival test. (B) Bacterial CFUs per gram tissue in Peyer’s Patches, MLN, Spleen and Liver in B6 and Card19^lxcn mice seven days post-oral infection with Yptb. Representative of two (PP) or four (MLN, Spleen, Liver) independent experiments. Unpaired Student’s t-test. ** p < 0.01, * p < 0.05. (C) Representative images of naive and Yptb-infected B6 and Card19^lxcn spleens, seven days post-infection Representative of three independent experiments. (D) Quantification of naive and infected spleen weights. Data are pooled from three independent experiments. Naive (n = 5), Yptb (B6 n = 19, Card19^lxcn n = 16) 2-way ANOVA with Bonferroni multiple comparisons post-test. ** p < 0.01. (E) Serum cytokines from naive and Yptb infected B6 and Card19^lxcn mice. Data are pooled from four independent experiments. Naïve (n = 8), Yptb (B6 n = 31, Card19^lxcn n = 28) 2-way ANOVA with Bonferroni multiple comparisons post-test. * p < 0.05, n.s. not significant.