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. 2021 Oct 12;11:748730. doi: 10.3389/fonc.2021.748730

Figure 4.

Figure 4

Loss of H3K4me2 increases GS expression in adipose cells. (A) Fold change of individual amino acid (AA) omitted from PC of WT and TgGS mice (n = 3, *P < 0.05, **P < 0.01). (B) Schematic diagram of methionine cycle. (C) The protein levels of GS, LSD1, H3K4me2, and H3 in non-treated or 5FU-treated mouse adipose cells cultured with NM or CT26 cell supernatants were measured with Western blot. (D) WT mice and TgGS mice were intraperitoneally inoculated with MC38 cells and treated with 5FU or 5FU+GSK-LSD1 for 2 weeks, respectively. Then, the representative images of the mic-bearing tumors were showed. Arrow heads, tumors. (E) The tumors in (D) were isolated and weighed (n = 5, **P < 0.01). (F) Kaplan-Meier curves for the survival of 5FU-treated or 5FU+GSK-LSD1-treated WT mice or TGGS mice after MC38 tumor cell inoculation plotted against time (days after injection) (n = 10, **P < 0.01).