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. Author manuscript; available in PMC: 2022 Oct 6.
Published in final edited form as: Neuron. 2021 Aug 13;109(19):3104–3118.e6. doi: 10.1016/j.neuron.2021.07.018

Fig. 2: Rnf43, but not Znrf3, is a target of Wnt in OPCs.

Fig. 2:

(A) Fold change of mRNA levels for Rnf43, Znrf3, Axin2 and Notum in cultured wildtype OPCs treated with varying concentrations of Wnt3a (marked on x axis) compared to no treatment (n=3 independent experiments). (B) Fold change of mRNA levels for Rnf43, Znrf3, Axin2 and Notum in cultured OPCs derived from Olig2cre:APCfl/fl mice compared to control APC fl/fl/mice (n=3 independent experiments. (C) Rnf43 immunohistochemistry (and co-staining with Pdgfrα) in cultured OPCs derived from Olig2cre:APCfl/fl mice compared to control APCfl/fl mice. Scale bar 50μm. (D) Protein levels by western blot of Rnf43 and Znrf3 in cultured OPCs, differentiated OL, and astrocytes (AST) from control APCfl/fl mice and in OPCs from Olig2cre:APCfl/fl mice. (E) Fold change of Rnf43 mRNA in cultured wildtype OPCs versus mature OL differentiated for 3 days (n=3 independent experiments). (F) Rnf43 immunohistochemistry in cultured PDGFRα expressing WT OPCs, and in differentiated OL expressing MBP after 3 days of switching to differentiation media. (G) Rnf43 protein fluorescent intensity is significantly increased in Olig2+ OPCs (arrowheads) from PDGFRα-creER:APC fl/fl mice compared to APC fl/fl control (non cre) mice at 5dpl following lysolecithin induced corpus callosum focal demyelination (Scale bar 20μ), with Rnf43 fluorescent intensity quantification in OPCs in (H). (I) Fold changes of total mRNA levels in 5dpl lesioned corpus callosum tissues of PDGFRα-creER:APC fl/fl mice compared to APC fl/fl control. Values in all panels are mean±s.d. NS not significant, *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.