Skip to main content
. Author manuscript; available in PMC: 2022 Oct 6.
Published in final edited form as: Neuron. 2021 Aug 13;109(19):3104–3118.e6. doi: 10.1016/j.neuron.2021.07.018

Fig. 3: Rnf43 and Znrf3 functions are dispensable for developmental myelination.

Fig. 3:

(A) MBP immunohistochemistry in multiple brain regions at postnatal day 10 (P10) and P14 in Olig1cre:RZ fl/fl (double Rnf43/Znrf3 floxed mice referred to as RZfl/fl) and RZfl/fl control (non cre) mice brains. Scale bar 1mm. (B) Fold change of MBP fluorescent intensities at P10 and P14 in Olig1cre:RZ fl/fl corpus callosum (CC) compared to RZfl/fl control (n=4 animals). (C, D) MAG+ mRNA expressing mature OL by in situ hybridization (C), and quantification of fold change (D)(n=4 animals), in Olig1cre:RZ fl/fl CC compared to RZfl/fl control. (E, F, G) Electron-microscopy (EM)(E), quantification of number of myelinated axons (F), and G-ratio calculations of myelinated axons (G) in P21 CC of RZfl/fl control and Olig1cre:RZ fl/fl. Scale bar 10μ in E. n=3 animals; The scatterplot in (G) displays g-ratios of individual axons as a function of axonal diameter. Values in all panels are mean±s.d. NS not significant, *p<0.05. See also Fig. S2 and S3.