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. Author manuscript; available in PMC: 2022 Oct 6.
Published in final edited form as: Neuron. 2021 Aug 13;109(19):3104–3118.e6. doi: 10.1016/j.neuron.2021.07.018

Fig. 4: Rnf43 function is critical for remyelination.

Fig. 4:

(A, B) MAG+ mRNA expressing OLs at 10 days post lesioning (dpl) and 21 dpl following lysolecithin induced corpus callosum focal demyelination in induceable PDGFRα-creER:RZfl/fl mice and RZfl/fl control (non cre) mice (lesioning schematic Fig. S4A), and (B) quantification of MAG+ cells in lesion areas. Dotted line marks lesion extent in corpus callosum. (C) DAPI staining and Olig2 immunohistochemistry identifies lesion area and oligodendroglial cell numbers in lesions respectively in the same mice as above, and (D) quantification of Olig2+ cells in lesion areas at 10dpl and 21dpl. (E) MBP Immunostaining in lesions at 21dpl in PDGFRα-creER:RZfl/fl and RZfl/fl control mice (enlargements shown in E’ and E”) with (F) quantification of MBP intensities at 10dpl and 21dpl in lesions. (G) Electron microscopy (EM) of remyelination at 14 dpl and 21 dpl in PDGFRα-creER:RZfl/fl and RZfl/fl/control mice, with (H) quantification of myelinated axons in lesions from these same mice (n=4 animals). (I) EM of remyelination at 50 dpl in PDGFRα-creER:RZfl/fl and RZfl/fl control mice. (J) G-ratio analyses of remyelination at 14dpl, 21dpl and 50dpl (scatterplot displays g-ratios of individual axons as a function of axonal diameter. n=4 animals) in PDGFRα-creER:RZfl/fl and RZfl/fl/ control lesions (solid lines show linear trend lines). (K) MAG+ mRNA expressing OLs at 14 dpl following lysolecithin induced corpus callosum focal demyelination in PDGFRα-creER:RZfl/fl double mutant mice, PDGFRα-creER:Rnf43 fl/fl single mutant mice, PDGFRα-creER:Znrf3 fl/fl single mutant mice, and RZfl/fl control mice (non cre), and (L) quantification of MAG+ cells in lesion areas. Values in all graphs are mean±s.d. NS not significant, *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. Scale bars in all images are 100μm, except in (G) which is 100μm. See also Fig. S4, S5 and S6.